top of page

Welcome to the Biomolecular Engineering laboratory at BME

Our strategic research concept is to create novel molecules through biomolecular engineering to develop biosensing technologies dedicated to health care management.

Ultimately, we aim to create innovative theranostic biomolecules and biodevices which can aid in the recognition and/or diagnosis of medical conditions and provide medicine/treatment to patients suffering from various diseases (metabolic disorders, mental disorders, cancer, etc.).

The targets for our biomolecular engineering are enzymes, antibodies, binding proteins, receptors/transporters, aptamers, and synthetic molecules. With the combination of a variety of electrochemical principles (amperometric, potentiometric, voltametric, and impedimetric) and advanced platforms, we are developing innovative biosensing technologies to drive the improvement of human health and quality of life.

Currently, the Sode lab is focusing on the development of:
1. The creation of engineered biosensing molecules (enzymes/binding proteins/antibodies/aptamers, 
2. The development of innovative in vivo, real-time, multi-parameter and continuous biosensing systems, 
3. Innovative POCT/single use sensing systems for biomarkers, 
4. The development of biomedical devices for closed-loop therapies.

What is "Biomolecular Engineering"?

Biomolecular engineering is the design and/or creation of new biomolecules and/or the mimicking of biomolecules based on their structural and functional information. Our strategic approaches toward biomolecular engineering are based on knowing the existing and/or future requirements for accurate and precise monitoring of biomarkers that are essential for medical and health care management. Engineering of biomolecules is realized by introducing strategically designed mutations, replacing or inserting novel structures, and utilizing fusion technologies as well as in silico predictions. The creation of novel biosensing principles is accomplished by designing novel biomolecules with expected features suitable for these new biosensing principles. The discovery, design, and creation of novel biomolecules is the most promising and attractive strategy to develop novel biosensors dedicated to the analytical demands from a variety of fields; medical, health, environment, process monitoring, etc. Our research targets of biomolecular engineering that are dedicated to the development of novel biosensing systems are enzymes, antibodies, receptors, binding proteins, aptamers, and synthetic molecules (polymers).

PI: Dr. Sode’s most notable research activities are biomolecular engineering research topics, represented by the development of engineered enzymes which can be used in self-monitoring glucose sensing systems for diabetes. Specifically, Dr. Sode has focused on a family of enzymes called glucose dehydrogenases (GDHs), which serve as a scaffold and a template enzyme molecule to construct an ideal enzyme for use in self-monitoring of blood glucose (SMBG) as well as in continuous glucose monitoring systems. (CGM). In particular, Dr. Sode has focused on engineering the following enzymes/proteins: glucose oxidase, glucose dehydrogenases, cholesterol oxidase, lactate oxidases, fructosyl amino acid/peptide oxidases, glucose binding proteins, and fructosyl amino acid kinase/binding proteins. These enzymes/proteins are engineered by introducing strategically designed amino acid substitutions and/or using chimeric enzyme/protein construction to improve their performance for a specific application. Structural elucidation of the biosensing molecules are carried out to obtain necessary information to exploit our biomolecular engineering approach. Our group has also expanded research activities to include the science and engineering of novel protein groups, natively unfolded proteins. Natively unfolded proteins, or disordered proteins, are a group of proteins that have little or no ordered structure under physiological conditions. In particular, our group has focused on α-synuclein, a protein attributed to cause Parkinson’s disease (PD). Studies on the mechanism of amyloid formation and the use of amyloid fibrils for bio-nanostructure scaffolds are also reported.  

Selected recent research articles about biosensing technologies

What is "Biosensors"?

Biosensors are defined as “analytical devices incorporating a biological material, a biologically derived material or a biomimic intimately associated with or integrated within a physicochemical transducer or transducing microsystem, which may be optical, electrochemical, thermometric, piezoelectric, magnetic or micromechanical” (Turner et al., 1987). The history of biosensors began with the invention of a glucose sensor by Professor Clark in 1956, which was constructed through the combination of a Clark type oxygen electrode and the legendary enzyme, glucose oxidase (GOx). The most characteristic and yet indispensable component in biosensors is the biologically derived or mimicked biosensing molecule i.e., enzymes, antibodies, aptamers, binding proteins, receptors, synthetic receptors, molecularly imprinted polymers, cells etc. Biosensors have been developed by applying existing biomolecules which have been used for the analyses and diagnosis, newly discovered biomolecules, and engineered and/or newly created biomolecules. Currently, the most advanced biosensors are the glucose sensors used for continuous glucose monitoring (CGM) systems. Our future goals are to construct biosensors which are operated continuously for real-time in vivo monitoring of target molecules and to combine these with sensor-augmented therapeutic devices to provide predicted target concentrations.

 

 


Biosensing Technology


Our group’s research topics in biosensing technologies include the development of enzyme/microbial sensors for diagnostic use, as well as for environmental and food monitoring. Most of these sensor systems employ novel enzymes that were engineered or isolated in our laboratory. These include direct-electron-transfer (DET) enzyme sensing systems employing protein-engineered enzymes and novel biosensing systems employing engineered antibodies, binding proteins, and artificial ligands, such as molecularly imprinted polymers (MIPs) and DNA aptamers. We are using various sensor platforms, including screen-printed electrodes, gold electrodes, interdigitated electrodes and gold micro-electrodes to construct innovative and practical electrochemical biosensing systems. Current most focused area is the development of in vivo continuous monitoring system, based on various modalities of bioelectrochemical sensors, employing our unique and superb engineered biosensing molecules. We also reported on the construction of a novel biofuel cell system employing engineered enzymes, a miniaturized biofuel cell that uses direct electron transfer type glucose dehydrogenase in the anodic catalyst and that could be used in combination with microsystems, designated as a “BioCapacitor”, which realizes a stand-alone, self-powered and autonomous wireless glucose sensing system. Current focus is the creation of bio- and environmental resorvable BioBatteries.

 

Selected recent research articles about biosensing technologies

OVERVIEW
What is "Biomolecular Engineering"?
What is Biosensors?
bottom of page