Publications

2026

Convenient and sensitive detection of viable Escherichia coli employing a sequential reaction between antibody-enzyme complexes

Shogo Izumi, Daimei Miura, Yasukazu Maeda, Koji SODE, Wakako Tsugawa, Kazunori Ikebukuro, Ryutaro Asano

Biosensors and Bioelecrtonics

in press

Rapid and convenient electrochemical hemoglobin detection in mouse feces employing a DNA aptamer to evaluate the severity of colitis in a mouse model

Mizuki Nakashima, Daimei Miura, Erika Komiya, Nanami Watanabe, Kaori Tsukakoshi, Tsutomu Omatsu, Wakako Tsugawa, Koji SODE, Kazunori Ikebukuro, Toshinori Yoshida, Ryutaro Asano

Analytical Methods

in press

Self-Adhesive Conductive Elastomers for Gel-Free Biopotential Recording

Kirstie M.K. Queener, Alec Brewer, He Sun, Jack Twiddy, Vladimir A. Pozdin, Koji SODE, Alper Bozkurt, Michael Daniele

Advanced Electronic Materials

in press

Creation of an Engineered Oxygen-Insensitive L-Glutamate Oxidase for the Application of Electrochemical L-Glutamate Sensors

Mika Hatada, Shouhei Takamatsu, Ryutaro Asano, Kazunori Ikebukuro, Wakako Tsugawa, Koji SODE

Int. J. Mol. Sci.

27(6), 2831

A bioreference electrode; a reference electrode which shifts its redox potential symmetrically with the working electrode to develop reliable bioelectrochemical sensors

Joseph A. Kerrigan (Jr.), David Probst, Koji SODE

Biosensors and Bioelectronics

304, 118619

A Unique Insertion Loop Facilitates Tight NAD+ Binding in Nicotinoprotein: Insights from In Vitro Loop Engineering and In Silico Studies

Houcheng Xue, Takumi Yanase, Junko Okuda-Shimazaki, Haruka Kawai, Daimei Miura, Ryutaro Asano, Kazunori Ikebukuro, Koji SODE, Wakako Tsugawa

Int. J. Mol. Sci.

27(5), 2367

Transient Analysis and Engineering of Bacterial Metabolic Pathways Using Cell-penetrating Peptide-Peptide Nucleic Acid Conjugates

Yugo Kawabuchi, Shunichi Kobayashi, Jaeyoung Yu, Gota Kasai, Miku Kobe, Rei Suo, Shiro Itoi, Koji SODE, Takahiro Muraoka, Ryutaro Asano, Tetsushi Mori

Microbial Cell Factories

25, 27

2025

Electrochemical Impedance Spectroscopy Analog Frontend Optimized for Mobile Biosensing Applications

Jack Twiddy, Ethan Cove, Chris Sharkey, Koji SODE, Michael Daniele

ECS Sensors Plus

19322968251384979

Development of an extended gate field effect transistor enzymatic sensor to monitor glucose in human plasma

David Probst, Jack Twiddy, Mika Hatada, Michael Daniele, Koji SODE

J Diabetes Sci Technol

19322968251384979

Construction and Characterization of a Novel Direct Electron Transfer Type Enzymatic Sensor Using Spermidine Dehydrogenase

Sheng Tong, Yuki Yaegashi, Mao Fukushi, Takumi Yanase, Junko Okuda-Shimazaki, Ryutaro Asano, Kazunori Ikebukuro, Madoka Nagata, Koji SODE, Wakako Tsugawa

Biosensors

15, 681

Electrochemical aptamer-based sensor for single-step quantification of glycated albumin in point-of-care diabetes and pre-diabetes management

Andrea Rescalli, David Probst, Mika Hatada, Madoka Nagata, Francesco Cellesi, Pietro Cerveri, Koji SODE

Biosens.Bioelectron

288, 117826

Challenges in Realizing Therapeutic Antibody Biosensing

Madoka Nagata, Ellie Dewese Wilson, Kazunori Ikebukuro, Koji SODE

Trends in Biotechnology

44, 2, 365-377

Engineered single chain variable fragments (scFvs) with improved pH-dependent kinetics for use in continuous biosensor applications

Ellie D. Wilson, David Probst, Mai Hamasaki, Miho Oda, Varun Kochar, Qianming Xu, Ayumi Tanaka, Hirobumi Suzuki, Ryutaro Asano, Koji Sode

RSC Adv.

15, 25337-25348

Functional and Structural Analyses of Diverse G-Quadruplex and Non-G-Quadruplex Structures Formed by Guanine-Rich Nucleic Acids: A Study on the Insulin Aptamer

Naoya Iwano, Taiji Oyama, Mizuki Tomizawa, Shintaro Inaba, Ryuma Mori, Yasumoto Nakazawa, Koji SODE, Kaori Tsukakoshi, Takayuki Nakano, Kazunori Ikebukuro

Small

2501336

Expressing and functionally evaluating anticancer bispecific antibody fragments using cyanobacterium Synechocystis sp. PCC6803 for sustainable production

Gota Kasai, Sayumi Shioya, Miku Kobe, Shunichi Kobayashi, Shouhei Takamatsu, Koki Makabe, Izumi Kumagai, Koji SODE, Ryutaro Asano

Biochemical Engineering

221, 109776

In-vivo continuous monitoring with biosensors based on engineered biological recognition elements: opportunities and challenges

Madoka Nagata, Koji SODE

Frontiers in Sensors

6:1579359

Expanding the Bioanalytical Application of β-Hydroxybutyrate Binding Proteins Through Characterization of Their Metabolite Interactions and Site-Directed Mutagenesis

Bryant J. Kane, Kyle V. Murphy, Koji SODE

Protein Science

34:e70129

The development and application of an engineered direct electron transfer enzyme for continuous levodopa monitoring

Kartheek Batchua, David Probsta, Takenori Satomura, John Younce, Koji SODE

npj Biosensing

2: 1

2024

Development of tetravalent antibody–enzyme complexes employing a lactate oxidase and the application to electrochemical immunosensors

Miho Oda. Kentaro Hiraka, Wakako Tsugawa, Kazunori Ikebukuro, Koji SODE, Ryutaro Asano

Biosensors and Bioelectronics

267, 1

An electrochemical biosensor for the detection of tuberculosis specific DNA with CRISPR-Cas12a and redox-probe modified oligonucleotide

Saman Taufiq, Madoka Nagata, Shah Rukh Abbas, Koji SODE

Heliyon

10, 23, 15, e40754

The Diabetes Technology Society Error Grid and Trend Accuracy Matrix for Glucose Monitors

David Klonoff et al. including Koji SODE

J.Diabetes Science and Technology

e40754

A versatile method to create an antibody/split-enzyme complex and its application to rapid, homogeneous, and universal electrochemical immunosensing system

Yuka Tobita, Kensukue Hirano, Daimei Miura, Yuma Hatano, Wakako Tsugawa, Kazunori Ikebukuro, Koji SODE, Ryutaro Asano

Advanced Sensor Research

2400112

Improvement of substrate specificity of the direct electron transfer type FAD-dependent glucose dehydrogenase catalytic subunit

Joseph A. Kerrigan Jr., Hiromi Yoshida, Junko Okuda-Shimazaki, Brenda Temple, Katsuhiro Kojima, Koji SODE

J.Biotechnol

395, 20, 170-179

Proximity-Unlocked Luminescence by Sequential Enzymatic Reactions from Antibody and Antibody/Aptamer (PULSERAA): A Platform for Detection and Visualization of Virus-Containing Spots

Daimei Miura, Wakana Hayashi, Kensuke Hirano, Ikkei Sasaki, Kaori Tsukakoshi, Hidehumi Kakizoe, Satomi Asai, Christopher J. Vavricka, Hitoshi Takemae, Tetsuya Mizutani, Wakako Tsugawa, Koji SODE, Kazunori Ikebukuro, Ryutaro Asano

Advanced Science

2403871

Potential of Enzymatically Synthesized Hemozoin Analog as Th1 Cell Adjuvant

Kazuaki Hoshi, Anh Thi Tram Tu, Miwako Shobo, Karin Kettisen, Lei Ye, Leif Bülow, Yoji Hakamata, Tetsuya Furuya, Ryutaro Asano, Wakako Tsugawa, Kazunori Ikebukuro, Koji SODE, Tomohiko Yamazaki

Nanomaterials

14, 1440

Current trends and challenges in the glycemic control technologies for patients with diabetes

David Probst, Koji SODE

Latest Trends in Blood Glucose Monitoring and Control, Chapter 3 (Japanese book)

Levodopa: From Biological Significance to Continuous Monitoring

David Probst, Kartheek Batchu, John Robert Younce, Koji SODE

ACS Sensors

Development of therapeutic single-chain antibody sensing system for bioprocess monitoring

Mai Hamasaki, Shouhei Takamatsu, Madoka Nagata, Ellie Wilson, Hirobumi Suzuki, Ayumi Tanaka, Kazunori Ikebukuro, Koji SODE, Ryutaro Asano

Biosensors and Bioelectronics

261, 116511

Towards monitoring of critical illness via the detection of histones with extended gate field-effect transistor sensors

Hayley Richardson, Jeffrey Barahona, Greg Medwig, Angela Johns, Lina M. Acosta Pérez, Koji SODE, Michael Daniele, Francis J. Miller, Edgar Lobaton, Spyridon Pavlidis

Biosensors and Bioelectronics: X

19, 100501

Discovery of Periplasmic Solute Binding Proteins with Specificity for Ketone Bodies - β-Hydroxybutyrate Binding Proteins -

Bryant J. Kane, Junko Okuda-Shimazaki, Madelyn M. Andrews, Joseph A. Kerrigan Jr., Kyle Murphy, Koji SODE

Protein Science

e5025

The 2.5th Generation Enzymatic Sensors Based on the Construction of Quasi-Direct Electron Transfer Type NAD(P)-Dependent Dehydrogenases

Kurea Ikegai, Junko Okuda-Shimazaki, Truc Thanh Tran, Mika Hatada, Ryutaro Asano, Kazunori Ikebukuro, Wakako Tsugawa, Koji SODE

Biosensors and Bioelectronics

255, 116219

Development of closed bipolar electrode based L-lactate sensor employing quasi-direct electron transfer type enzyme with cyclic voltammetry

David Probst, Koji SODE

Biosensors and Bioelectronics

254, 116197

Direct Electron Transfer–Type Oxidoreductases for Biomedical Applications

Keisei Sowa, Junko Okuda-Shimazaki, Eole Fukawa, Koji SODE

Annual Reviews of Biomedical Engineering

Rapid and Convenient Single-chain Variable Fragments-Employed Electrochemical Detection System for C-Reactive Protein Applicable to Point-of-care Testing

Daimei Miura, Saki Motohashi, Ayaka Goto, Hayato Kimura, Wakako Tsugawa, Koji SODE, Kazunori Ikebukuro, Ryutaro Asano

International Journal of Molecular Science

25(5), 2859

Development of a glycated albumin sensor employing dual aptamer-based extended gate field effect transistors

Mika Hatada, Spyridon Pavlidis, Koji SODE

Biosensors and Bioelectronics

251, 116118

Development of direct electron transfer type extended gate field effect transistor enzyme sensors for metabolite detection

David Probst, Jack Twiddy, Mika Hatada, Spyridon Pavlidis, Michael Daniele, Koji SODE

Analytical Chemistry

Development of the BioBattery: a novel enzyme fuel cell using a multicopper oxidase as an anodic enzym e

Kartheek Batchu, David Probst, Takenori Satomura, Koji SODE

Biosensors and Bioelectronics

252, 116092

Development of an electrochemical impedance spectroscopy immunosensor for insulin monitoring employing pyrroloquinoline quinone as an ingestible redox probe

Mukund Khanwalker, Mika Hatada, Jeffery T. LaBelle, Koji SODE

Biosensors and Bioelectronics

250, 116049

Exploration and Application of DNA-Binding Proteins to Make a Versatile DNA−Protein Covalent-Linking Patch (D-Pclip): The Case of a Biosensing Element

Erika Komiya, Shouhei Takamatsu, Daimei Miura, Kaori Tsukakoshi, Wakako Tsugawa, Koji SODE, Kazunori Ikebukuro, and Ryutaro Asano

Journal of the American Chemical Society

146, 6, 4087–4097

2023

In vivo continuous monitoring of peptides and proteins: Challenges and opportunities

Ellie Wilson, David Probst, Koji Sode

Applied Physics Review

10(4), 041309

Development of interdigitated electrode based enzyme sensor for triglyceride monitoring using engineered oxygen insensitive glycerol 3-phosphate oxidase

Inyoung Lee, Sheng Tong, Mika Hatada, Adrianna Masterson, Kentaro Hiraka, Junko Okuda-Shimazaki, Wakako Tsugawa, Gary Hughes, Koji SODE

Sensors and Actuators Reports

5, 100164

Development of an Anti-Idiotype Aptamer-Based Electrochemical Sensor for a Humanized Therapeutic Antibody Monitoring

Madoka Nagata, Jinhee Lee, Taro Saito, Kazunori Ikebukuro, Koji SODE

International Journal of Molecular Sciences

24(6), 5277

Development of a versatile method to construct direct electron transfer-type enzyme complexes employing SpyCatcher/SpyTag system

Takumi Yanase, Junko Okuda-Shimazaki, Ryutaro Asano, Kazunori Ikebukuro, Koji SODE, Wakako Tsugawa

International Journal of Molecular Sciences

24(3), 1837

Surface area independent response of closed bipolar electrodes

David Probst, Inyoung Lee, Jeffrey E. Dick, Koji SODE

Sensors and Actuators Reports

5:100138

2022

Microgravity environment grown crystal structure information based engineering of direct electron transfer type glucose dehydrogenase

Junko Okuda-Shimazaki, Hiromi Yoshida, Inyoung Lee, Katsuhiro Kojima, Nanoha Suzuki, Wakako Tsugawa, Mitsugu Yamada, Koji Inaka, Hiroaki Tanaka, Koji SODE

Communications Biology

5:1334

Structure of lactate oxidase from Enterococcus hirae revealed new aspects of active site loop function: product inhibition mechanism and oxygen gatekeeper

Kentaro Hiraka, Hiromi Yoshida, Wakako Tsugawa, Ryutaro Asano, Jeffrey T. La Belle, Kazunori Ikebukuro, Koji Sode

Protein Science

31:10, e4434

The development of micro–sized enzyme sensor based on direct electron transfer type open circuit potential sensing principle

David Probst, Inyoung Lee, Koji SODE

Electrochimica Acta

426:10, 140798

A Thiol-Reactive Phenazine Ethosulfate (trPES) – A Novel Redox Mediator for Quasi-Direct Electron Transfer Type Sensors

Maya Fitriana, Kentaro Hiraka, Kazunori Ikebukuro, Koji SODE, Wakako Tsugawa

Sensors and Materials Sensors and Materials

34:6, 2105-2124

In vitro continuous 3 months operation of direct electron transfer type open circuit potential based glucose sensor: heralding the next CGM sensor

nyoung Lee, Wakako Tsugawa, Kazunori Ikebukuro, Koji SODE

Journal of Diabetes Science and Technology

16(5):1107-1113

Development of an electrochemical impedance spectroscopy based biosensor for detection of ubiquitin C-Terminal hydrolase L1

Jinhee Lee, Bryant Kane, Mukunc Khanwalker, Koji SODE

Biosensors and Bioelectronics

208:114232

A Glycemia Risk Index (GRI) of Hypo- and Hyperglycemia for Continuous Glucose Monitoring Validated by Clinician Ratings

David Klonoff et al. (multiple authors including Koji SODE)

Journal of Diabetes Science and Technology

doi: 10.1177/19322968221085273

An Amine-Reactive Phenazine Ethosulfate (arPES) - A Novel Redox Probe for Electrochemical Aptamer based Sensor

Jinhee Lee, Madoka Nagata, Stephen Henley, Kazunori Ikebukuro, Koji SODE

Sensors (MDPI)

24;22(5):1760

Development of a DNA aptamer that binds to the complementarity-determining region of therapeutic monoclonal antibody and affinity improvement induced by pH-change for sensitive detection

Taro Saito, Yutaka Shimizu, Kaori Tsukakoshi, Koichi Abe, Jinhee Lee, Kinuko Ueno, Ryutaro Asano, Brian V. Jones, Tomohiro Yamada, Tatsuki Nakano, Jiaxing Tong, Asami Hishiki, Kodai Hara, Hiroshi Hashimoto, Koji SODE

Biosensors and Bioelectronics

203:114027

Light‑induced production of isobutanol and 3‑methyl‑1‑butanol by metabolically engineered cyanobacteria

Shunichi Kobayashi, Shota Atsumi, Kazunori Ikebukuro, Koji SODE, Ryutaro Asano

Microbial Cell Factories

21:7

Transient potentiometry based D-serine sensor using engineered D-amino acid oxidase showing quasi-direct electron transfer property

Shouhei Takamatsu, Inyoung Lee, Jinhee Lee, Ryutaro Asano, Wakako Tsugawa, Kazunori Ikebukuro, Jeffrey E. Dick, Koji SODE

Biosensors and Bioelectronics

200 :113927

Development of a POCT type insulin sensor employing anti-insulin single chain variable fragment based on faradaic electrochemical impedance spectroscopy under single frequency measurement

Mukund Khanwalker, Rinko Fujita, Jinhee Lee, Ellie Wilson, Kohei Ito, Ryutaro Asano, Kazunori Ikebukuro, Jeffrey LaBelle, Koji SODE